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PETITE INDUCTION IN YEAST, Saccharomyces cerevisiae , BY PHOTOACTIVATION OF 3‐A‐ZIDO‐6‐A‐MINO‐10‐M‐ETHYLACRIDINIUM CHLORIDE
Author(s) -
Iwamoto Y.,
Yanagihara Y.,
Yielding L. W.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb09505.x
Subject(s) - saccharomyces cerevisiae , yeast , oxygen , chloride , irradiation , chemistry , dna damage , dna , saccharomyces , biochemistry , adduct , biophysics , reactive oxygen species , covalent bond , photochemistry , biology , organic chemistry , physics , nuclear physics
— The photoinduction of petite colonies and cell toxicity in non‐growing yeast, Saccharomyces cerevisiae , by 3‐a‐zido‐6‐a‐mino‐10‐m‐ethylacridinium chloride (AAMAC) has been examined. The results presented here indicate that mitochondrial DNA damage in resting yeast which occurs following irradiation of AAMAC‐treated cells for short time periods is probably mediated through a covalent adduct between AAMAC and DNA. Furthermore, the photoreaction which contributes to biological activity is dependent on the presence of oxygen. Pre‐irradiated AAMAC, which no longer exhibited the short‐term photo‐induction of biological effects showed a second biological activity. In this case longer irradiation times, e.g. 30 min, were required to induce petites for resting yeast. Again there was a strong dependence on the presence of oxygen. These results suggest that both processes may be effected through oxygen intermediates (photodynamic processes).