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WAVELENGTH DEPENDENCE FOR ORNITHINE DECARBOXYLASE INDUCTION IN VIVO †
Author(s) -
Kligman Lorraine H.,
Kaidbey Kays H.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb05641.x
Subject(s) - ornithine decarboxylase , hairless , in vivo , chemistry , stimulation , epidermis (zoology) , monochromator , irradiation , action spectrum , dose dependence , wavelength , biophysics , endocrinology , biology , biochemistry , enzyme , optics , anatomy , physics , nuclear physics , microbiology and biotechnology
— The wavelength dependence for ornithine decarboxylase induction was investigated in vivo in the epidermis of albino hairless mice. A 4 cm 2 area of dorsal skin was exposed to narrow wavebands (HPBW 6.6 nm) from a monochromator optically coupled to a 5 kW Xe arc source. Dose‐response studies were caried out at 260, 275, 290, 300 and 315 nm. The animals were sacrificed 24 h after irradiation and the epidermis separated and assayed for ODC activity. A dose‐related induction of ODC was observed at 260, 275, 290 and 300 nm. No stimulation was observed following exposure to 315 nm (maximal dose 6400 J/m 2 ). ODC induction appeared to be linearly related to log UV dose within the dose range of 200 to 1600 J/m 2 . The dose required to induce an approximately 50‐fold increase in activity (1.5 nmol CO 2 /h/mg protein) was determined from the dose‐response regression lines and used to construct an action spectrum. Peak effectiveness occurred at 290 nm, with a rapid loss after 300 nm. After correction for epidermal transmission, peak effectiveness was shown to occur around 260 nm.