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KINETICS OF PHOTOREACTIVATION AND LIQUID‐HOLDING RECOVERY IN YEAST CELLS
Author(s) -
Johnson Ronald G.,
Haynes Robert H.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb05623.x
Subject(s) - photolyase , kinetics , yeast , irradiation , radiosensitivity , ultraviolet , biology , chemistry , biophysics , biochemistry , materials science , dna repair , physics , optoelectronics , dna , quantum mechanics , nuclear physics
— A method is presented for analyzing data on the kinetics of photoreactivation and liquid‐holding recovery in microorganisms. The method extracts, from measurements on survival, the number of repairable lethal hits per cell remaining after a period of photoreactivation or liquid‐holding recovery. A semilogarithmic plot of this quantity as a function of the duration of the survival‐enhancing treatment reveals the nature of the kinetics of inactivation of the lethal hits. The method has been applied to photoreactivation and liquid‐holding recovery in yeast cells with wild‐type radiation resistance. In the case of photoreactivation of ultraviolet‐damaged cells, the number of lethal hits per cell is found to decrease exponentially with the length of exposure to a continuous source of photoreactivating light. Liquid‐holding recovery in cells exposed to ultraviolet light or X‐rays also results in exponential decrease of lethal hits with increasing time. The method is compared with the fluence‐decrement method and Dulbecco's treatment of photoreactivation data.