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EXTENSIVE CROSSLINKING BETWEEN SUBUNITS OF OLIGOMERIC PROTEINS INDUCED BY FUROCOUMARINS PLUS UV‐A IRRADIATION
Author(s) -
Schiavon O.,
Veronese F. M.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb05600.x
Subject(s) - furocoumarins , furocoumarin , chemistry , singlet oxygen , phototoxicity , moiety , enzyme , photochemistry , covalent bond , alcohol dehydrogenase , catalase , biochemistry , oxygen , stereochemistry , organic chemistry , in vitro , dna
— Exposure of oligomeric proteins to UV‐A irradiation in the presence of various furocoumarins results in extensive crosslinking of the enzyme subunits. The mechanism by which these covalent bridges are formed does not involve the furocoumarin moiety as the crosslinking agent, but depends upon oxidative processes, mainly involving singlet oxygen and radical mediated processes not involving oxygen. The formation of crosslinks is not per se responsible for the inactivation of enzymes since in glutamate dehydrogenase the two reactions proceed at the same rate, in catalase it is not accompanied by enzyme inactivation and in alcohol dehydrogenase the inactivation rate is higher than polymerization.