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WAVELENGTH DEPENDENCE FOR THE INDUCTION OF BACTERIOPHAGE LAMBDA BY ANTITUMOR AGENT GILVOCARCIN V
Author(s) -
Elespuru Rosalie K.,
Hitchins Victoria M.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb04716.x
Subject(s) - prophage , bacteriophage , lambda phage , monochromator , wavelength , lambda , hairless , fluence , lac operon , bacteria , escherichia coli , chemistry , physics , microbiology and biotechnology , biology , optics , gene , genetics , irradiation , nuclear physics
The wavelength dependence for the induction of a lambda‐ lacZ fusion phage of E. coli by photo‐activated gilvocarcin V was determined using a spot test or a quantitative assay for the lacZ gene product. Suspensions of bacteria and chemical were exposed to radiation of different wavelengths in the region 313‐546 nm, using a double grating monochromator. The prophage induction profiles generated were similar to the absorption spectrum of gilvocarcin V in this region, with a peak near 400 nm. The radiation fluence and chemical concentration required for threshold levels of prophage induction exhibited a nearly reciprocal relationship. These results have implications for the therapeutic use of gilvocarcins as antitumor agents.