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INACTIVATION OF PHAGE BY NEAR‐ULTRAVIOLET RADIATION AND HYDROGEN PEROXIDE
Author(s) -
Eisenstark A.,
Buzard R. L.,
Hartman P. S.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb04715.x
Subject(s) - hydrogen peroxide , nucleic acid , dna , rna , chemistry , ultraviolet radiation , genome , ultraviolet , bacteria , biology , microbiology and biotechnology , biochemistry , genetics , gene , physics , quantum mechanics , radiochemistry
A series of phage with different genomes (both single‐stranded and double‐stranded RNA and DNA) was inactivated with hydrogen peroxide (H 2 O 2 ) in various combinations with far‐ultraviolet (FUV) and near‐ultraviolet (NUV) radiations. In every case but one (a lipid‐coated phage), a sublethal H 2 O 2 concentration greatly enhanced killing by NUV but not FUV. Moreover, this NUV/H 2 O 2 synergism was oxygen independent and there was little if any host cell reactivation upon NUV plus H 2 O 2 inactivation. These results suggest that these phage are inactivated by a common mechanism irrespective of nucleic acid composition, but that some phage genomes may be more vulnerable to NUV/H 2 O 2 inactivation than others.