Premium
SITES OF PHOTOSENSITIZATION BY DERIVATIVES OF HEMATOPORPHYRIN
Author(s) -
Kessel David
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb04697.x
Subject(s) - intracellular , hematoporphyrin , incubation , chemistry , membrane , in vitro , l1210 cells , biochemistry , biophysics , cytotoxicity , biology , photodynamic therapy , organic chemistry
Leukemia L1210 cells were incubated in vitro with the tumor‐localizing product HPD (hem‐atoporphyrin derivative) for 0.5. 4 and 18 h. Effects of subsequent irradiation on viability, membrane transport and integrity, DNA synthesis and intracellular ATP concentration were assessed. Intracellular porphyrin pools were analyzed by HPLC. A 30 min incubation led to concentration of a readily‐exchangeable pool of monomeric HPD components at plasma membrane loci; irradiation resulted in photodamage to membrane transport and a loss in capacity for dye exclusion. In contrast, increasing the incubation time led to a corresponding increase in the size of a non‐exchangeable intracellular pool of other HPD components. Subsequent irradiation led to depletion of intracellular ATP and loss of capacity for biosynthesis of DNA, but little plasma membrane damage.