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A MICROSPECTROFLUOROMETRIC STUDY OF PORPHYRIN‐PHOTOSENSITIZED SINGLE LIVING CELLS—II. METABOLIC ALTERATIONS
Author(s) -
Kohen E.,
Reyftmann J. P.,
Morliere P.,
Santus R.,
Kohen C.,
Mangel W. F.,
Dubertret L.,
Hirschberg J. G.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb04694.x
Subject(s) - nad+ kinase , microinjection , chemistry , metabolic pathway , porphyrin , mitochondrion , pentose phosphate pathway , hematoporphyrin , biochemistry , biology , metabolism , biophysics , microbiology and biotechnology , enzyme , glycolysis , photodynamic therapy , organic chemistry
The transient change in the NAD(P)H NAD(P) equilibrium following microinjection of mitochondrial (malate) or extra‐mitochondrial (6‐phosphogluconate) substrates into single living cells is strongly modified a few seconds after the onset of 365 nm irradiation in the presence of hematoporphyrin. There is no major difference in the time frame for the alterations of the Krebs cycle and pentose pathway. In view of the complex interrelationships between mitochondrial and extramitochondrial pathways, there is a reasonable chance that effects on both pathways are not unrelated‐ However there is no definite evidence for a direct correlation between the photoeffects on these two pathways.