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CELL SURFACE DAMAGE IN CULTURED MAMMALIAN CELLS WITH SYNCHROTRON RADIATION AT 160 nm
Author(s) -
Shinohara Kunio,
Ito Atsushi,
Ito Takashi
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb04684.x
Subject(s) - trypsinization , irradiation , synchrotron radiation , hela , membrane filter , membrane , biophysics , chemistry , materials science , cell , optics , biology , biochemistry , physics , enzyme , trypsin , nuclear physics
Cultured mammalian cells (HeLa) were allowed to attach onto a membrane filter and were irradiated with 160 nm synchrotron radiation. The cells then were rinsed with medium, fixed, and stained. Some of the cells became detached from the membrane filter during irradiation before post‐irradiation incubation at 37°C. The cells remaining attached to the membrane filter were released by trypsinization, collected and examined for dye‐exclusion ability with eosin Y. The number of stained cells was increased when the cells were irradiated at 160 nm, while no such increase was observed in cells irradiated with synchrotron radiation at 220 nm, with a low pressure Hg lamp (predominantly 254 nm), or with gamma‐rays of 60 Co. These results indicated that the cell surface was injured by irradiation with synchrotron radiation at 160 nm.