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SINGLE‐STRAND BREAKS IN SUPERCOILED DNA INDUCED BY VACUUM‐UV RADIATION IN AQUEOUS SOLUTION
Author(s) -
Takakura Kaoru,
Ishikawa Mitsuo,
Hidea Kotaro,
Kobayashi Katsumi,
Ito Atsushi,
Ito Takashi
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb04682.x
Subject(s) - aqueous solution , photodissociation , radical , dna supercoil , dna , chemistry , photochemistry , radiation , absorption (acoustics) , biophysics , molecule , wavelength , materials science , optics , physics , biology , biochemistry , optoelectronics , organic chemistry , dna replication
We studied the induction of single‐strand breaks in the DNA of plasmid pBR 322 by vacuum‐UV radiation above 145 nm in aqueous solutions in relation to the production of OH‐radicals in water. The similarity and dissimilarity were examined of the wavelength dependence between the two effects. The maximum of single strand breaks at 150 nm could be explained by the action of OH‐radicals derived from direct water photolysis: the maximum at 180 nm remains unexplained. There was no indication that the direct absorption of photon by the DNA molecule plays an important role in the production of single‐strand breaks.