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LASER FLASH PHOTOLYSIS STUDIES OF DNA‐COMPLEXED ETHIDIUM BROMIDE
Author(s) -
Atherton Stephen J.,
Beaumont Paul C.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb03570.x
Subject(s) - ethidium bromide , flash photolysis , molar absorptivity , chemistry , bromide , singlet state , photochemistry , fluorescence , absorption (acoustics) , laser , excited state , photodissociation , dna , materials science , optics , inorganic chemistry , atomic physics , reaction rate constant , kinetics , biochemistry , physics , quantum mechanics , composite material
— Laser flash photolysis studies of DNA‐complexed ethidium bromide were undertaken. We have observed a singlet‐singlet (S 1 ‐S n ) absorption process for DNA‐complexed ethidium bromide. The observed lowest singlet excited state lifetime was 21 ± 2 ns. The molar difference extinction coefficient was measured to be 2.4 ± 0.4 × 10 3 M ‐1 cm ‐1 at 370 nm. The assignment of this transition was confirmed by time resolved fluorescence measurements.