Premium
EFFECT OF MID‐UV RADIATION ON DNA‐Cu 2+ COMPLEX: ABSORPTION AND CIRCULAR DICHROISM STUDY
Author(s) -
Matzeu Mirella,
Onori Giuseppe
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb03564.x
Subject(s) - circular dichroism , absorption (acoustics) , irradiation , dna , copper , chemistry , absorption spectroscopy , photochemistry , crystallography , dichroism , absorption band , analytical chemistry (journal) , ion , range (aeronautics) , materials science , optics , chromatography , biochemistry , organic chemistry , composite material , physics , nuclear physics
— When DNA is complexed with Cu 2+ ions it becomes sensitized to mid‐UV light. Irradiation of the complex at 310 ± 10 nm produces marked changes in absorption and circular dichroism spectra in addition to those in melting profiles of irradiated samples. These variations show conformational changes in DNA structure due to a photoreduction of some copper sites in the DNA‐Cu 2+ complex. In addition to this effect an irreversible damage in DNA structure could be invoked to explain the observed behaviour. These facts can be related to the presence in DNA‐Cu 2+ complex of a small absorption band extending up to mid‐UV range while the absorption of DNA is negligible in this spectral range.