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THE CYTOTOXIC AND MUTAGENIC EFFECTS OF UVA RADIATION ON L5178Y MOUSE LYMPHOMA CELLS
Author(s) -
Hitchins V. M.,
Withrow T. J.,
Olvey K. M.,
Harleston B. A.,
Ellingson O. L.,
Bostrom R. G.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb03563.x
Subject(s) - fluence , mutagen , lymphoma , microbiology and biotechnology , cytotoxic t cell , cytotoxicity , cancer research , chemistry , biology , irradiation , immunology , in vitro , genetics , dna , physics , nuclear physics
— A broad‐band UVA source that emits primarily350–400 nm radiation and no measurable radiation below 340 nm was used to test toxicity and mutagenicity at the thymidine kinase locus in L5178Y, subclone 3.7.2C (TK + / ‐ ) mouse lymphoma cells. Cells were exposed to a fluence of 0 to 80 × 10 4 J/m 2 . The relationship between UVA fluence and survival was found to have a shoulder region followed by an exponential decrease in survival at higher fluence levels. An exposure‐dependent increase in mutation was observed with increasing fluences from 0 to about 60 × 10 4 J/m 2 . An approximately 3‐ to 4‐fold increase in mutations (trifluorothymidine resistance) over unexposed, control cells was seen at a fluence that resulted in 90% cell killing. We conclude that UVA radiation is a mutagen in the L5178Y mouse lymphoma cells used in this study.