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REPAIR IN Escherichia coli OF A PSORALEN‐DNA INTERSTRAND CROSSLINK SITE SPECIFICALLY INTRODUCED INTO T 410 A 411 OF THE PLASMID pUC 19
Author(s) -
Zhen WeiPing,
Jeppesen Claus,
Nielsen Peter E.
Publication year - 1986
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1986.tb03562.x
Subject(s) - escherichia coli , plasmid , biology , transversion , dna , chemistry , microbiology and biotechnology , genetics , mutation , gene
— Escherichia coli was transformed with a pUC 19 plasmid which contained a 4,5′,8‐trimethylpsoralen cross‐link specifically at position T 410 ‐A 411 , and transformants in which the cross‐link had been repaired were scored on the basis of ampicillin resistance. Repair was only seen after induction of the SOS‐system by shortwave ultraviolet irradiation. The repair efficiency was 6%, and of 100 colonies analyzed only two was found to be mutated at the cross‐linked site: a T‐A transversion was observed at position 410. A repair mechanism which does not involve recombination is proposed.