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INCORPORATION OF THE SPIN TRAP DMPO INTO CULTURED FETAL MOUSE LIVER CELLS
Author(s) -
Morgan David D.,
Mendenhall Charles L.,
Bobst Albert M.,
Rouster Susan D.
Publication year - 1985
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1985.tb03552.x
Subject(s) - spin trapping , hydrogen peroxide , chemistry , electron paramagnetic resonance , saline , fetus , spin (aerodynamics) , microbiology and biotechnology , radiochemistry , photochemistry , nuclear magnetic resonance , biochemistry , radical , biology , pregnancy , physics , endocrinology , genetics , thermodynamics
— The incorporation of four different spin traps into cultured fetal mouse liver cells was investigated using electron spin resonance spectroscopy. The cells were incubated in saline solutions of the traps, washed, and then irradiated in a saline solution containing hydrogen peroxide. The presence of a reproducible ESR signal was taken as evidence of spin trap incorporation. The spin trap DMPO was found to be incorporated, while PBN and 4‐POBN were not. MNP was toxic to the cells.