Premium
TIME‐RESOLVED FLUORESCENCE STUDIES OF SPINACH CHLOROPLASTS–EVIDENCE FOR THE HETEROGENEOUS BIPARTITE MODEL
Author(s) -
Berens Sylvia J.,
Scheele Jurgen,
Butler Warren L.,
Magde Douglas
Publication year - 1985
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1985.tb03547.x
Subject(s) - fluorescence , quenching (fluorescence) , spinach , chloroplast , chemistry , photochemistry , bipartite graph , biophysics , range (aeronautics) , steady state (chemistry) , biology , materials science , physics , biochemistry , optics , computer science , gene , graph , theoretical computer science , composite material
— Fluorescence lifetimes of spinach chloroplasts were measured with a modelocked dye laser and time‐correlated single photon counting. Information about energy transport and functional organization of the chloroplasts is revealed by such time‐resolved fluorescence studies. Quenching experiments using treatment with UV light or the chemical agent dibromothymoquinone are consistent with the notion that there is heterogeneity associated with PS II units and that such heterogeneity is reflected over the entire time range of fluorescence decay, not just in a single component. Phosphorylation experiments were also carried out which permit us to relate these kinetic studies to previous steady state observations.