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VACUUM‐ULTRAVIOLET PHOTOACOUSTIC SPECTROSCOPY OF BIOLOGICAL MATERIALS USING SYNCHROTRON RADIATION AS A LIGHT SOURCE
Author(s) -
Inagaki Takashi,
Ito Atsushi,
Motosuga Masatsugu,
Hieda Kotaro,
Kobayashi Katsumi,
Maezawa Hiroshi,
Ito Takashi
Publication year - 1985
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1985.tb03522.x
Subject(s) - optics , synchrotron radiation , materials science , radiation , wavelength , signal (programming language) , photoacoustic effect , absorptance , photoacoustic imaging in biomedicine , optoelectronics , physics , reflectivity , computer science , programming language
— Photoacoustic measurements of biological materials such as DNA, lysozyme, squalene and yeast cells were performed for the first time in the vacuum‐UV region above 150 nm using the gas‐microphone technique and synchrotron radiation as a light source. The samples studied were in a form of thin film supported on a self‐supporting collodion film. The results obtained for the phase of the photoacoustic signal were found consistent with the predictions from the simple one‐dimensional theory. The signal amplitudes obtained as a function of the incident wavelength were interpreted as giving the absorptance (the fraction of the incident photon energy dissipated in the sample) spectrum.