Premium
RED: FAR‐RED RATIO DOES NOT MODULATE THE ABUNDANCE OF TRANSCRIPTS FOR TWO MAJOR CHLOROPLAST POLYPEPTIDES IN LIGHT‐GROWN Pisum sativum TERMINAL SHOOTS *
Author(s) -
Jenkins G. I.,
Smith H.
Publication year - 1985
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1985.tb01630.x
Subject(s) - pisum , far red , phytochrome , sativum , biology , shoot , botany , epicotyl , chloroplast , rubisco , photosynthesis , chlorophyll , biochemistry , red light , gene
— We have used specific, cloned cDNA hybridisation probes to measure the abundance of transcripts for the small subunit (SSU) of ribulose bisphosphate carboxylase‐oxygenase, and the major apoprotein (LHCP) of the light‐harvesting chlorophyll alb complex, in total RNA extracts of Pisum sativum shoots grown under different light qualities. The plants were grown in continuous white light of a constant fluence rate (50 p.mol m −2 s −1 ;400–700 nm) supplemented with far‐red light to give different ratios of red: far‐red light, and hence different in vivo phytochrome photoequilibria. Variation of the red: far‐red ratio over the range 1.87 to 0.10, calculated to give in vivo P fr : P total ratios of 0.78 to 0.37, had no significant effect on leaf expansion but had a marked effect on internode extension rate. The abundances of the SSU and LHCP RNAs in RNA extracts from the terminal shoot were not significantly different in the four light quality treatments. The results are discussed in relation to the photoregulation of expression of these genes in light‐grown plants.