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RESOLUTION OF OVERLAPPING BANDS IN THE NEAR‐UV ABSORPTION SPECTRUM OF INDOLE DERIVATIVES
Author(s) -
Ragone Raffaele,
Colonna Giovanni,
Servillo Luigi,
Irace Gaetano
Publication year - 1985
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1985.tb01601.x
Subject(s) - indole test , chromophore , ethylene glycol , chemistry , derivative (finance) , absorption spectroscopy , absorption (acoustics) , spectral line , photochemistry , resolution (logic) , molecular electronic transition , solvent , second derivative , computational chemistry , stereochemistry , molecule , organic chemistry , physics , optics , mathematics , quantum mechanics , mathematical analysis , financial economics , economics , artificial intelligence , computer science
— The second derivative spectra of tryptophan in water and in ethylene glycol at 22°C have been integrated in order to obtain the corresponding primitive functions. The integration was carried out by making use of Tchebychev polynomials. The results show that the integrated primitive functions do not correspond to the original absorption spectra of tryptophan in various solvents, but they reflect only the contributions of the 1 L b bands of the indolic chromophore. The identification of the electronic component, which generates the second derivative spectrum, was based on the solvent insensitivity of the derivative peaks. The comparison between the absorption spectra reported in this paper and those calculated for the 1 L b ← 1 A electronic transition of indole confirmed the assumption that the derivation process eliminates the broad, although more intense, contributions coming from the 1 L a ← 1 A electronic transition.