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THE USE OF SHORT LIVED FLUORESCENT DYES TO CORRECT FOR ARTIFACTS IN THE MEASUREMENTS OF FLUORESCENCE LIFETIMES
Author(s) -
Mauzeraix D.,
Ho P. P.,
Alfano R. R.
Publication year - 1985
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1985.tb01558.x
Subject(s) - fluorescence , photomultiplier , ultrashort pulse , wavelength , optics , triphenylmethane , excitation , materials science , microchannel plate detector , pulse (music) , photodetector , relaxation (psychology) , detector , optoelectronics , chemistry , physics , laser , psychology , social psychology , organic chemistry , quantum mechanics
— The ultrafast emission for fluorophores can be used as the effective excitation pulse for prompt response measurements by photo detectors in the same spectral region as that of unknown samples. This method corrects for such artifacts as wavelength and spatial dependence of the response function of the photodetector. It is shown that the emission from triphenylmethane dyes is an excellent effective pulse with relaxation time 2 ps in the red region of the spectrum. A microchannel plate photomultiplier has only a 35 ps increase in response or lag time between excitation at 420 nm and emission at 680 nm.