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DIFFERENT (DIRECT and INDIRECT) MECHANISMS FOR THE INDUCTION OF DNA‐PROTEIN CROSSLINKS IN HUMAN CELLS BY FAR‐ and NEAR‐ULTRAVIOLET RADIATIONS (290 and 405 nm)
Author(s) -
Peak Meyrick J.,
Peak Jennifer G.,
Jones Carol A.
Publication year - 1985
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1985.tb01552.x
Subject(s) - dna , irradiation , chemistry , biophysics , reactive oxygen species , ultraviolet radiation , oxygen , dna damage , biochemistry , photosensitizer , photochemistry , biology , radiochemistry , organic chemistry , physics , nuclear physics
— Apparent DNA‐protein crosslinking induced by monochromatic 290 and 405 nm Tadiations was measured in cultured human P3 teratocarcinoma cells with DNA alkaline elution techniques. The rates of the induction of crosslinks by 290 nm radiation were the same when the cells were irradiated either aerobically or anaerobically or when the cells were in an H 2 O or D 2 O aqueous environment. With 405 nm radiation, anaerobic irradiation reduced the induction of the crosslinks (dose modifying factor is about 0.2), and about twice as many crosslinks were observed when the cells were irradiated in an environment of D 2 O rather than H 2 O. The results are consistent with the hypothesis that far‐UV radiation induces DNA‐protein crosslinks by a direct mechanism, whereas near‐UV radiation induces crosslinks via indirect photodynamic photosensitizations in which unidentified cellular endogenous photosensitizers and reactive species of oxygen are used.