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COMPARISON OF EFFECTS OF UVA, UVB AND UVC ON GROWTH AND VIABILITY OF MITOGEN‐STIMULATED HUMAN PERIPHERAL BLOOD CELLS
Author(s) -
PRETELL JUDITH O.,
WIMBERLY JOANNE,
MCAULIFFE DANIEL J.,
PARRISH JOHN A.
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb08193.x
Subject(s) - lymphoblast , thymidine , peripheral blood mononuclear cell , dna synthesis , cell culture , andrology , microbiology and biotechnology , population , biology , immunology , cell growth , in vitro , chemistry , biochemistry , medicine , genetics , environmental health
— Peripheral blood mononuclear cells were irradiated with UVA, UVB or UVC. The highest exposure dose used in each waveband reduced the number of viable cells to one‐third the control cell population after 3 days in culture. Exposure of these cells to half as much UV from each waveband resulted in an equivalent or greater degree of inhibition of their proliferative response to mitogen as measured by lymphoblast transformation, [ 3 H]‐thymidine uptake and viable cell number on day 3 in culture. The pattern of inhibition was distinct for each waveband. UVA interfered with blastogenesis on the first 2 days of culture at doses which had considerably less effect on viable cell number. UVA also depressed the first round of DNA synthesis, which was detectable on the second day of culture. By day 3 in culture, however, the UVA‐induced reduction in both the number of lymphoblasts and the uptake of [ 3 H]‐thymidine was a direct reflection of reduced numbers of viable cells. UVB did not interfere with blastogenesis in mitogen‐stimulated cultures to the same degree as did UVA. Only the highest dose of UVB depressed blast transformation more than viable cell number on day 1; by day 2 lower doses were also inhibitory. In contrast UVC had little effect on blastogenesis at any time; a reduced number of lymphoblasts observed on days 2 and 3 in culture was a direct reflection of a reduced number of viable cells rather than a reduced percent of these cells undergoing blast transformation. As with UVA‐irradiated, mitogen‐stimulated cells, [ 3 H]‐thymidine uptake was also depressed in both UVB and UVC irradiated, mitogen‐stimulated cells on day 2. However, only UVB continued to depress DNA synthesis more than viable cell number after 3 days of culture. These results suggest that UVA, UVB and UVC may interfere with any one or more of the signals involved in the response to mitogen, be they the recognition of mitogen by T cells or accessory cells, the transformation of lymphocytes into lymphoblasts or the activation of lymphoblasts to synthesize DNA.