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DETECTION OF DNA‐PSORALEN PHOTOADDUCTS in situ
Author(s) -
ZAREBSKA ZOFIA,
JARZABEKCHORZELSKA MARIA,
RZȨSA GENOWEFA,
GLIŃSKI WIESLAW,
PAWIŃKA MARIA,
CHORZELSKI TADEUSZ,
JABLOŃKA STEFANIA
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb08182.x
Subject(s) - in vivo , dna , in vitro , microbiology and biotechnology , hairless , chemistry , fluorescence , psoralen , substrate (aquarium) , in situ , biophysics , biology , biochemistry , ecology , physics , organic chemistry , quantum mechanics
— An immunological method, with the use of specific immune serum, has been developed for detection of 8‐methoxypsoralen (8‐MOP) photoadducts to DNA, formed in situ in cell nuclei, after combined treatment with 8MOP and UV‐A irradiation (Zarçbska et al. , 1978). Lymphocytes fixed on slides or in suspension, and cryostat sections of different mammalian tissues, served as antigenic substrate, after treatment with 8‐MOP and UV‐A in vitro. Specific fluorescence in these substrates was detected in the nuclei after treatment with 30 ˜ 140 kJ/m 2 UV‐A in the presence of 0.1‐0.3 μg/cm 2 8‐MOP. PHA‐stimulated‐lymphocytes appeared to be the most sensitive substrate. However, hairless mice treated with high doses of UV‐A in vivo , 70 ˜ 360 kJ/m 2 did not reveal a specific fluorescence of epidermal nuclei, unless a high local concentration of 8‐MOP was attained. The apparent discrepancy in the level of photoadduct detection between the in vitro and in vivo treated specimens was explained by the low number of DNA‐8‐MOP‐photoadducts formed in vivo under these experimental conditions. The relevance of these findings to the role of DNA‐8‐MOP‐photoadducts formed during PUVA photochemotherapy is discussed.