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EFFICIENCY OF REPAIR OF PYRIMIDINE DIMERS AND PSORALEN MONOADDUCTS IN NORMAL AND XERODERMA PIGMENTOSUM HUMAN CELLS
Author(s) -
Cleaver James E.,
Charles Wayne C.,
Kong Seouk Hwan
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb05350.x
Subject(s) - xeroderma pigmentosum , psoralen , pyrimidine dimer , aphidicolin , nucleotide excision repair , dna , dna damage , dna repair , ultraviolet light , microbiology and biotechnology , dna polymerase , chemistry , biology , biochemistry , photochemistry
—Repair of DNA damage produced by ultraviolet light or 5‐methylisopsoralen in normal and xeroderma pigmentosum human cells involves many similar steps. Aphidicolin and cytosine arabinoside block repair of both kinds of damage with similar efficiency, indicating that DNA polymerase a has a major role in repair for these lesions. In xeroderma pigmentosum cells of various complementation groups, the relative efficiency of excision repair for both ultraviolet‐ and 5‐methylisopsoralen‐induced damage was group A < C < D, indicating a close resemblance between both kinds of lesions in relation to the repair deficiencies in these groups. At high doses, the maximum rate of repair of damage by ultraviolet light was about twice that for methylisopsoralen damage, possibly because ultraviolet‐induced damage forms a substrate that is more readily recognized and excised than that of the psoralen adducts. Differences in the structural distortions to DNA caused by these kinds of damage could be detected using single strand specific nucleases which excised dimers but not 5‐MIP adducts from double strand DNA.