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PROPERTIES OF A NEW STATE OF HEMATOPORPHYRIN IN DILUTE AQUEOUS SOLUTION
Author(s) -
Ricchelli Fernanda,
Grossweiner Leonard I.
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb05347.x
Subject(s) - hematoporphyrin , chemistry , micelle , aqueous solution , triton x 100 , porphyrin , photochemistry , metal , liposome , monomer , copper , fluorescence , divalent , inorganic chemistry , organic chemistry , polymer , photodynamic therapy , pulmonary surfactant , biochemistry , physics , quantum mechanics
—Dilute aqueous hematoporphyrin undergoes a transformation after standing at room temperature leading to a shift of the Soret peak from 395 nm to 405 nm, disappearance of visible bands I (610 nm) and IV (503 nm), a shift of the first emission band from 615 nm to 580 nm, and fluorescence polarization at room temperature. The transformation was accelerated by heating and was faster for more dilute solutions. It did not take place in ethanol, 99:1 glycerol‐water, or in small egg lecithin liposomes. It was rapid in SDS micelles and did not occur in CTAB and Triton X‐100 micelles. Low concentrations of divalent zinc and copper increased the rate of transformation, but the product was not the corresponding metal ion ligand. The transformation was fast with uroporphyrin I and fast but incomplete with hematoporphyrin derivative. The methyl esters of hematoporphyrin IX and uroporphyrin I did not transform. The tentative identification of the transformed product is a tightly‐bound linear micelle, oriented such that the central hydrogens of the free base monomer units are anti‐parallel.