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USE OF SYNTHETIC POLYNUCLEOTIDES TO CHARACTERISE AN ANTISERUM MADE AGAINST UV‐IRRADIATED DNA
Author(s) -
L. Mitchell David,
Clarkson Judith M.
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb04646.x
Subject(s) - antiserum , polynucleotide , dna , antigenicity , chemistry , adduct , irradiation , circular dichroism , microbiology and biotechnology , affinity chromatography , dna damage , biochemistry , antibody , biology , enzyme , organic chemistry , genetics , physics , nuclear physics
UV—irradiated single‐stranded synthetic homopolymers were used to characterise an antiserum made against UV‐irradiated DNA. Using induction data obtained by reversed‐phase high performance liquid chromatography, the relative antigenicity of the polymers could be converted to relative binding affinity for each DNA photoproduct. Since no Pyr(6–4)Pyo adducts could be detected at 320 nm under these conditions, the affinity of the antiserum for this lesion could be compared with that for cyclobutane dimers. The relative binding capacity of Thy(6–4)Thy: Thy(5–6)Thy: Cyt(6–4)Cyt: Cyt(5–6)Cyt = 166: 1: 0.25: 0.062.