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QUANTITATION OF THE PHOTOMUTAGENIC RESPONSE OF Salmonella TO AN EASTERN SHALE OIL AND FLUORESCENT LIGHT
Author(s) -
Selby Christopher,
Calkins John,
Enoch Harry G.
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb04594.x
Subject(s) - oil shale , shale oil , reversion , fluorescence , auxotrophy , chemistry , salmonella , radiation , histidine , strain (injury) , food science , radiochemistry , biophysics , biochemistry , biology , bacteria , genetics , escherichia coli , optics , physics , gene , anatomy , phenotype , paleontology , amino acid
The dose‐response relationship for photomutation (i.e. photosensitized mutation) by a shale‐derived oil was examined. The Ames’ Salmonella typhimurium tester strain TA98 was exposed to several concentrations of an Eastern shale oil and UV‐visible radiation from illumination‐type fluorescent lamps. Reversion to histidine prototrophy and survival were assayed following various radiation doses. Reciprocity of shale oil concentration and radiation exposure over approximately 10‐fold ranges of oil concentrations and radiation doses was observed with revertant numbers per plate, percent survival, and the induced frequency of revertants (revertants per survivor). The relationship between mutation frequency and the product (shale oil concentration times radiation exposure) fit well with either a linear model or a power law model in which the frequency of induced mutations was described by the product dose raised to the 1.26th power. Similar dose‐response relationships provide potential criteria for comparing potency of photomutagenic substances, comparisons that may be valuable towards assessing, and perhaps modifying, risks imposed by human exposure to synthetic fuels.