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GENETIC VARIATIONS AND LINOLENIC ACID INDUCED CHANGES IN THE ORIENTATION PATTERN OF CHLOROPHYLL a IN THYLAKOID MEMBRANES
Author(s) -
Szitó T.,
Kiss J. G.,
Garab GY. I.,
Mustárdy L. A.,
FaludiDániel Á.
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb04561.x
Subject(s) - thylakoid , fluorescence , chlorophyll , fluorescence anisotropy , absorbance , chemistry , membrane , chlorophyll a , photochemistry , biophysics , chlorophyll fluorescence , absorption (acoustics) , chloroplast , biology , biochemistry , botany , chromatography , optics , physics , gene
Orientation pattern of the Q y absorption and emission dipoles of chlorophyll a were studied in wild type Scenedesmus obliquus and in mutants deficient in chlorophyll b and carotenoids. Fluorescence polarization ratio at –140°C and linear dichroism at 25°C were measured in whole cells and thylakoids aligned in polyacrylamide gel. Unlike normal thylakoids, mutants displayed fluorescence polarization ratios significantly lower than 1.0 and showed a negative LD signal around 672 nm, indicating the tendency of the Q y dipoles to tilt out from the membrane plane. Such an orientation pattern can also be artificially induced by treating normal thylakoids with linolenic acid.