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CHEMILUMINESCENCE INDUCED BY OXIDATION OF TRYPTOPHAN BY SINGLET OXYGEN AND BY HYPOCHLOROUS ACID. IMPLICATIONS IN THE LUMINESCENCE EMITTED IN PHAGOCYTOSIS
Author(s) -
Violet P. Fornier de,
Veyret B.,
Vincendeau P.,
Caristan A.
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb03913.x
Subject(s) - chemiluminescence , hypochlorous acid , singlet oxygen , phagocytosis , opsonin , chemistry , zymosan , tryptophan , photochemistry , light emission , luminescence , oxygen , myeloperoxidase , reactive oxygen species , biochemistry , biophysics , amino acid , organic chemistry , in vitro , microbiology and biotechnology , biology , materials science , immunology , inflammation , optoelectronics
— The mechanism of the chemiluminescence emitted during phagocytosis of opsonized zymosan was investigated. The use of an artificial system generating singlet oxygen permitted the distinction between the respective roles of O 2 and HOCl in the light production via the reactions with opsonized zymosan, amino‐acids and peptides. The reaction of free hypochlorous acid, produced by the myeloperoxidase H 2 O 2 ‐Cl‐ system, with free tryptophan appears to be a likely path for the production of chemiluminescence. Implications for the mechanism of in vivo chemiluminescence during phagocytosis are considered.