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THE LETHAL EFFECT OF LONGWAVE ULTRAVIOLET LIGHT AND PUVA. AN ANALYSIS BASED UPON HUMAN MESENCHYMAL CELLS IN VITRO
Author(s) -
Jongh G. de,
BERGERS MIEKE,
Boezeman J. B. M.,
Verhagen A. R.,
Mier P. D.
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb03901.x
Subject(s) - phototoxicity , in vivo , mesenchymal stem cell , dermis , in vitro , chemistry , ultraviolet radiation , ultraviolet , irradiation , biophysics , microbiology and biotechnology , biology , materials science , biochemistry , anatomy , radiochemistry , physics , optoelectronics , nuclear physics
— The lethal effect of UVA and PUVA radiation was studied in cultures of fresh and mature monocytes. UVA radiation alone was shown to possess a lethal effect at doses which are attained in the dermis in vivo. The synergistic action of 8‐methoxypsoralen and UVA radiation predominated in PUVA radiation, but again a residual effect of UVA alone was demonstrated mathematically. Mature cells were less sensitive than fresh monocytes. Our results indicate that a monolayer culture of non‐dividing, mesenchymal cells offers considerable advantages over in vivo systems as a model for the study of phototoxicity.