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MODIFYING FACTORS OF THE CELLULAR CONCENTRATION OF PHOTOLYASE MOLECULES IN Saccharomyces cerevisiae CELLS–I. EFFECTS OF TEMPERATURE AND LIGHT
Author(s) -
Fukui Atsushi,
Laskowski Wolfgang
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb03899.x
Subject(s) - saccharomyces cerevisiae , cycloheximide , photolyase , yeast , biophysics , plasmid , biology , chemistry , biochemistry , microbiology and biotechnology , gene , dna repair , protein biosynthesis
— The effects of temperature and light on the cellular concentration of photoreactivating enzyme (PRE) molecules in haploid Saccharomyces cerevisiae cells were investigated. (1) Temperature effect: The number of active PRE molecules per cell (N PRE ) in cells grown at 37°C was about 13% of that grown at 23°C, although the amount of proteins per cell remained the same. (2) Light effect: N PRE in cells grown in light was about 2.8 times larger than that grown in the dark. The value of N PRE in cells grown in the light decreased more rapidly during holding in buffer in the dark than in the light. The N PRE decrease during holding in buffer in the dark was more rapid in cells grown in the light than grown in the dark. A comparable decrease was observed after holding in buffer in the presence of cycloheximide. (3) In cells harboring a plasmid containing the gene PHR1, N PRE was larger in cells grown at 23 than at 30°C.