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RED/FAR‐RED PHOTOREVERSIBILITY: NOT AN APPROPRIATE PHYTOCHROME ASSAY FOR RED‐LIGHT PREIRRADIATED CORN COLEOPTILES
Author(s) -
SCHMIDT WERNER
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb03437.x
Subject(s) - phytochrome , coleoptile , far red , red light , absorption (acoustics) , biology , wavelength , optics , biophysics , botany , physics
— Based on measurements with a single beam spectrophotometer, it has been found that subsequent red/far red irradiation cycles, which are usually given to monitor phytochrome content by dual wavelength spectroscopy, induce chlorophyll‐related absorption changes in maize coleoptiles. Therefore, the difference signal, usually measured between 730 and 800 nm or 660 and 730 nm after saturating red and far red irradiations, does not represent solely the phytochrome content of preirradiated samples.