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THE PRIMARY REACTION IN THE PHOTOREDUCTION OF PROTOCHLOROPHYLLIDE MONITORED BY NANOSECOND FLUORESCENCE MEASUREMENTS
Author(s) -
BOCHOVE A. C.,
GRIFFITHS W. T.,
GRONDELLE R.
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb03411.x
Subject(s) - protochlorophyllide , fluorescence , nanosecond , chemistry , photochemistry , analytical chemistry (journal) , enzyme , biochemistry , optics , chromatography , laser , physics , biosynthesis
— Time resolved fluorescence measurements, carried out on protochlorophyllide reductase enriched membranes from oat ( Avena sativa ), are described. A fast (1 ns at 293 K) decaying fluorescence component is assigned to the photoactive NADPH‐protochlorophyllide‐enzyme complex, while a slower (5 ns) component is ascribed to non‐photoactive protochlorophyllide. The results are interpreted in terms of a new fast primary step in the light requiring step of chlorophyll synthesis. The temperature dependence of the rate of this reaction has been studied by measuring the decay time of the fast fluorescence component at various temperatures from 77 to 293 K. Complete spectra of the kinetic fluorescence components have been measured at 293, 160 and 77 K.