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DOXYCYCLINE INDUCED PHOTODAMAGE TO HUMAN NEUTROPHILS AND TRYPTOPHAN
Author(s) -
SANDBERG SVERRE,
GLETTE JOHAN,
HOPEN GUNNAR,
SOLBERG CLAUS OLA
Publication year - 1984
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1984.tb03402.x
Subject(s) - doxycycline , singlet oxygen , chemistry , catalase , chemiluminescence , superoxide dismutase , photosensitivity , reactive oxygen species , tryptophan , superoxide , mannitol , photochemistry , photobiology , tetracycline , irradiation , oxygen , azide , biochemistry , biology , enzyme , antibiotics , physics , botany , organic chemistry , amino acid , quantum mechanics , nuclear physics
— Neutrophil functions were studied following irradiation (340–380 nm) of the cells in the presence of 22 mU M doxycycline. At increasing light fluence the locomotion, chemiluminescence and glucose oxidation (by the hexose monophosphate shunt) of the neutrophils steadily decreased. The photodamage increased with increasing preincubation temperature and time and was enhanced in D 2 O, reduced in azide and abolished in anaerobiosis. Superoxide dismutase, catalase or mannitol did not influence the photodamage. Photooxidation of tryptophan in the presence of doxycycline was increased9–10‐fold in D 2 O and nearly abolished in the presence of 0.25 m M NaN 3 , indicating that singlet oxygen is the most important reactive oxygen species in the doxycycline‐induced photodamage. The results may explain some of the features of tetracycline‐induced photosensitivity and why other authors have obtained diverging results when studying the influence of tetracyclines on neutrophil functions.