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PREPARATION AND PROPERTIES OF CHLOROPHYLL/WATER‐SOLUBLE MACROMOLECULAR COMPLEXES IN WATER. STABILIZATION OF CHLOROPHYLL AGGREGATES IN THE WATER‐SOLUBLE MACROMOLECULE
Author(s) -
Inamura Isamu,
Ochiai Hideo,
Toki Kenzi,
Watanabe Sachiko,
Hikino Satomi,
Araki Takeo
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb08363.x
Subject(s) - macromolecule , polyethylene glycol , polyvinylpyrrolidone , polymer , polyvinyl alcohol , aqueous solution , chemistry , peg ratio , chlorophyll , bovine serum albumin , chemical engineering , chromatography , polymer chemistry , organic chemistry , biochemistry , finance , engineering , economics
— As an artificial model compound of the chlorophyll‐protein complex in vivo , the chlorophyll/water‐soluble macromolecular complexes were prepared by using synthetic linear polymers of polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), polyethylene glycol (PEG), and a natural polymer of bovine serum albumin (BSA). By the method described here, it is possible to prepare an aqueous chlorophyll (Chl)‐macromolecular complex solution of a desired Chi aggregate, such as: Chi a (670), Chi a (740) and Chi b ; and with a desired relative content and concentration. These procedures for preparing such complexes will have wide applicability for technical use in Chi studies. For example, extremely diluted aqueous complex solutions of at least 1 × 10 ‐4% wt Chi a (670 or 740)‐macromolecular complex / wt can be obtained without changing the spectral features. From viscosity measurements, the structures of the complexes were inferred: (1) for a linear macromolecular (PVA or PVP) complex, a Chi species is tightly surrounded by a chain of the polymer causing shrinkage of the chain; (2) globular BSA molecules surround Chi species to form a large complex. The mechanism of stabilization of Chi aggregates in thylakoid membrane was discussed concerning an analogy to the complexes studied here.