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PROTOPORPHYRIN‐INDUCED PHOTODAMAGE: STUDIES USING CULTURED SKIN FIBROBLASTS
Author(s) -
Latham Patricia S.,
Bloomer Joseph R.
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb04517.x
Subject(s) - protoporphyrin , erythropoietic protoporphyria , protoporphyrin ix , endoplasmic reticulum , chemistry , fibroblast , cell damage , programmed cell death , cell culture , cell , apoptosis , microbiology and biotechnology , biochemistry , biophysics , biology , photodynamic therapy , in vitro , porphyrin , genetics , organic chemistry
A new system is described for the study of protoporphyrin‐induced photodamage of cells. This system differs from those previously described in that fibroblasts are induced to synthesize protoporphyrin from its precursor δ‐aminolevulinic acid. Fibroblasts were cultured from skin biopsies of 6 normal individuals and 6 patients with protoporphyria. All cell lines in both groups accumulated protoporphyrin when incubated with δ‐aminolevulinic acid in the absence of iron. Irradiation for 2 min with long‐wave UV light caused death of cells which had accumulated protoporphyrin, but not of cells which had been incubated without δ‐aminolevulinic acid. Cell damage could be quantitatively assessed by the release of chromium‐51 into the medium. Examination of irradiated protoporphyrin‐rich fibroblasts by electron microscopy revealed no significant differences between lines from patients with protoporphyria and normal individuals. The earliest indications of photodamage were rarifaction of the mitochondrial matrix with dilatation of cristae. dilatation of endoplasmic reticulum, and loss of plasma membrance integrity. Condensation of the cells correlated with cell death. These structural alterations suggest a generalized cellular injury.