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INTRODUCTION OF PYRIMIDINE DIMERS INTO DIFFERENT INTRACELLULAR FORMS OF SIMIAN VIRUS 40
Author(s) -
Edenberg Howard J.,
Roman Ann
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb04476.x
Subject(s) - thymidine , chromatin , dna , intracellular , virus , endonuclease , simian , pyrimidine dimer , pyrimidine , microbiology and biotechnology , chemistry , bacteriophage , biology , dna synthesis , biophysics , biochemistry , virology , dna repair , escherichia coli , gene
We examined the production of pyrimidine dimers by UV radiation in different intracellular forms of simian virus 40 DNA. Virus and chromatin or previrions were selectively labeled with [ l4 C]‐thymidine and [ 3 H]‐thymidine, respectively, in the same monolayer of infected cells. Viral DNA was extracted immediately after irradiation, and pyrimidine dimers were detected as sites sensitive to the UV‐endonuclease encoded by bacteriophage T4. No difference in the number of dimers introduced into chromatin, previrions. or virions was detected.