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USE OF THE DYE HOECHST 33258 TO INCREASE THE PHOTOSENSITIVITY OF BROMODEOXYURIDINE‐CONTAINING CELLULAR SLIME MOLDS
Author(s) -
Graetzer R.,
Deering R. A.
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb04465.x
Subject(s) - photosensitivity , bromodeoxyuridine , dictyostelium discoideum , ultraviolet , chemistry , biophysics , slime mold , photochemistry , cell growth , biochemistry , biology , materials science , optoelectronics , gene
The photosensitivity of amoebae of Dictyostelium discoideum to near‐ultraviolet light (near‐UV; 365 nm peak) was investigated following growth in 5‐bromodeoxyuridine (BrdUrd) and treatment with the dye Hoeschst 33258. Cell killing was studied as a function of dye‐treatment time and concentration, and BrdUrd incorporation time and concentration. For example, cells grown for 13 h in 50 μg BrdUrd/ml and then treated for 1 h in 10 μg dye/ml were reduced to 10% survival by a near‐UV exposure of 0.8 min. Combinations of any two of the three factors (BrdUrd, dye, near‐UV) were ineffective for killing. The sensitivity of the cells to near‐UV decreased rapidly as the holding time between the end of the dye treatment and the start of the UV exposure was increased.