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THE ROLE OF 4‐THIOURIDINE IN LETHAL EFFECTS AND IN DNA BACKBONE BREAKAGE CAUSED BY 334 nm ULTRAVIOLET LIGHT IN Escherichia coli
Author(s) -
Peak M. J.,
Peak J. G.,
Nerad Lisa
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb04453.x
Subject(s) - escherichia coli , dna , ultraviolet , monochromatic color , chromophore , dna damage , strain (injury) , ultraviolet light , chemistry , biology , photochemistry , biophysics , biochemistry , materials science , optoelectronics , gene , botany , anatomy
Strains of Escherichia coli that lack 4‐thiouridine ( 4 Srd) are killed by monochromatic 334 nm UV light (UV) less efficiently than their wild‐type parents, which contain 4 Srd. Oxygen enhancement ratios (OER) at 10% survival are 3.3 for a strain that possesses 4 Srd, and 2.6 for one that lacks 4 Srd. Single‐strand breaks in DNA caused by 334 nm UV accumulate more than twice as fast in the wild‐type strains than in the strains lacking 4 Srd. The results suggest that 4 Srd is an important chromophore in some near‐UV lethal effects. The results also suggest that the excitation energy from 334 nm UV light may be passed from RNA to DNA, resulting in single‐strand breaks.