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BINDING OF ANTHRACENE TO CELLULAR MACROMOLECULES IN THE PRESENCE OF LIGHT
Author(s) -
Sinha Birandra Kumar,
Chignell Colin F.
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb04430.x
Subject(s) - anthracene , chemistry , catalase , dna , macromolecule , lipid peroxidation , covalent bond , biophysics , superoxide dismutase , photochemistry , oxygen , biochemistry , irradiation , denaturation (fissile materials) , nuclear chemistry , enzyme , organic chemistry , biology , physics , nuclear physics
Ultraviolet radiation (δ > 295 nm) induced covalent binding of anthracene to DNA which increased with time and was not affected by oxygen. Irradiation in the presence of anthracene induced nicking of Col E, circular DNA and decreased the thermal denaturation temperature of calf thymus DNA. These effects were oxygen dependent, and were decreased by GMP. Irradiation of anthracene and human serum albumin resulted in covalent binding of the hydrocarbon to the protein accompanied by crosslinking of the protein. Protein crosslinking decreased under anaerobic conditions. Irradiation of anthracene bound to liposomes induced lipid peroxidation which was not affected by superoxide dismutase or catalase.