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RIBOFLAVIN SENSITIZED PHOTOREDUCTION OF NITRO BLUE TETRAZOLIUM ION (NBT 2+ ) IN DEGASSED AQUEOUS SOLUTION
Author(s) -
Takakubo M.,
Faure J.
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb03854.x
Subject(s) - riboflavin , chemistry , aqueous solution , reagent , nitro , ion , reaction rate constant , photochemistry , kinetics , superoxide , photodissociation , formazan , inorganic chemistry , nuclear chemistry , organic chemistry , enzyme , biochemistry , alkyl , physics , quantum mechanics
— When sensitized by riboflavin, the reduction of NBT 2+ (nitro blue tetrazolium ion) takes place in both aerated and degassed aqueous solution. Laser photolysis experiments revealed that the triplet riboflavin reduces NBT 2+ to nitro blue tetrazolinyl radical, NBT 2+ . and it disappears according to pseudo‐first order kinetics, with a bimolecular rate constant of (4.2 ± 0.2) X 10 8 M ‐1 s ‐1 . It is shown that NBT 2+ is not always a good detecting reagent for the superoxide anion, when the formation of the anion is mediated by riboflavin.