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PHYTOCHROME CONTROL OF ITS OWN SYNTHESIS IN Sorghum vulgare AND Avena sativa
Author(s) -
Otto V.,
Mousinger E.,
Sauter M.,
Schäfer E.
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb03602.x
Subject(s) - phytochrome , avena , darkness , phytochrome a , red light , biology , hordeum vulgare , far red , reticulocyte , etiolation , botany , rna , mutant , poaceae , biochemistry , arabidopsis thaliana , enzyme , gene
— The accumulation of phytochrome in the dark was measured for Avena sativa seedlings after a white light pretreatment and for Sorghum vulgare seedlings after continuous red or far‐red light treatments, using the herbicide Norflurazon to prevent greening under continuous irradiation. In both cases the accumulation of phytochrome depends on the state of the phytochrome at the light‐dark transition: high P fr levels (red light pulse) led to a slower rate of phytochrome accumulation than lower P fr levels (long wavelength far‐red (RG 9) light pulse). Poly‐(A + )‐RNA was isolated fromA. sativa seedlings grown for 48 h in darkness + 24 h WL + light pulse (5 min) (red, RG 9 light, red followed by RG 9 light or RG 9 followed by red light pulse) + 19 h darkness. The poly‐(A + )‐RNA was translated in a rabbit reticulocyte lysate system and the translation products were immunoprecipitated by specific anti‐phytochrome antibodies. It was demonstrated that the activity of mRNA coding for phytochrome was under phytochrome control.