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PHOTOTOXIC EFFECTS OF FOUR PSORALENS ON L1210 CELLS. THE CORRELATION WITH DNA INTERSTRAND CROSS‐LINKING
Author(s) -
Nielsen Peter Eigil,
Bohr Vilhelm
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb03596.x
Subject(s) - psoralen , phototoxicity , dna , chemistry , l1210 cells , hydroxymethyl , dna synthesis , furocoumarin , in vitro , dna damage , viability assay , biochemistry , stereochemistry , cytotoxicity
— L1210 mouse leukaemia cells were treated with psoralen [S‐methoxy‐(XMOP), 4,5′,8‐trimethyI‐(TMP), 4′‐hydroxymethyl‐4,5′,8‐trimethyl‐(HMT) or 4′‐amino‐ methyl‐4,5′,8‐trimethylpsoralen (AMT)] in combination with long wavelength ultraviolct irradiation (Λ∼ 365 nm). In order to investigate the relative photobiological activities of the psoralens, cell viability and DNA‐synthesis activity as well as psoralen‐DNA photoaddition and DNA interstrand cross‐linking were measured after the treatment. In all assays the activity ranking order was found to he: TMP > HMT > AM7 > 8MOP. Furthermore, a direct correlation between phototoxicity, psoralen induced DNA interstrand cross‐links and inhibition of DNA synthesis was indicated. Finally, psoralen uptake by the cells appears to be an important determinant for phototoxicity, whereas their DNA photoreactivity does not.