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THE ACTION OF ULTRAVIOLET RADIATION ON THE CELL PROLIFERATION OF TWO‐CELL MOUSE EMBRYOS
Author(s) -
Endoh H.,
Domon M.
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb03381.x
Subject(s) - embryo , somatic cell , cell cycle , cell , biology , microbiology and biotechnology , cell growth , irradiation , dna synthesis , cell division , dna , radiation , cell culture , ultraviolet radiation , biophysics , chemistry , genetics , optics , radiochemistry , physics , nuclear physics , gene
— The two‐cell mouse embryo has a unique cell cycle of a short DNA synthesis (S) phase and an extremely long post‐DNA synthesis (G 2 ) phase. An attempt was made to investigate the radiation biology of the long G 2 phase using UV radiation as a probe. Two cell mouse embryos, at various positions in the cell cycle, were UV‐irradiated in phosphate‐buffered saline. The embryos were cultured for a few hours to 3 days to assay for their cell proliferative characteristics. The embryos were most sensitive to the killing action of UV radiation in the late G 2 phase. The embryos divided more than two times after low UV fluences before dying and experienced G 2 phase delays. These results can be contrasted to the situation in somatic cells, in which the action of UV radiation is S phase selective. One possibility is that the target for the action of UV radiation is different in two‐cell mouse embryos from that in somatic cells and that the target is similar to that for X‐ray effects.