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UV‐INDUCED ELEVATION OF SKIN GLYCOSAMINOGLYCAN LEVELS I: RAPID MEASUREMENT OF SKIN GLYCOSAMINOGLYCANS BY TITRATION WITH ACRIDINE ORANGE *
Author(s) -
Menter Julian M.,
Pearl William S.,
Yadven Mitchell W.,
Willis Isaac
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb03371.x
Subject(s) - acridine orange , glycosaminoglycan , hairless , titration , chemistry , fluorescence , orange (colour) , acridine , chromatography , biochemistry , organic chemistry , apoptosis , physics , food science , quantum mechanics
— We have developed a simple, rapid, sensitive procedure for measuring polyanionic glycosaminoglycans (GAG) in unfixed defatted lyophilizedSK–1 hairless mouse skin by means of quantitative measurement of uptake of the fluorescent dye acridine orange (AO), which was shown to be GAG‐specific under the conditions of these experiments. This method is suitable for detecting and estimating elevations in steady skin GAG levels due to UV irradiation.