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TRIFLUOPERAZINE: CORNEAL ENDOTHELIAL PHOTOTOXICITY
Author(s) -
Hull David S.,
Csukas Steve,
Green Keith
Publication year - 1983
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1983.tb03362.x
Subject(s) - trifluoperazine , phototoxicity , ultraviolet light , biophysics , perfusion , chemistry , ultraviolet , pharmacology , in vitro , medicine , biochemistry , biology , materials science , photochemistry , optoelectronics , calmodulin , enzyme
— Trifluoperazine is a commonly used agent for the treatment of psychiatric disorders. Perfusion of corneal endothelial cells with trifluoperazine‐HCI concurrent with 3 min of exposure to long wavelength ultraviolet light resulted in a corneal swelling rate which was greater than that found in corneas where endothelial cells were perfused with trifluoperazine‐HCI and not exposed to ultraviolet light. Exposure of endothelial cells to 25 W incandescent light for 5 min during perfusion with trifluoperazine‐HCI did not result in a corneal swelling rate in excess of that found during perfusion with trifluoperazine in the dark. The increased corneal swelling rate could be produced by pre‐exposure of the trifluoperazine‐HCI perfusing solution to ultraviolet light suggesting the production of toxic photo‐products during exposure of trifluoperazine HCI to ultraviolet light. Perfusion of corneal endothelial cells with non‐ultraviolet illuminated trifluoperazine HCI had no effect on endothelial cell membranes or ultra‐structure. This is in contrast to cells perfused with trifluoperazine HCI that had been exposed to ultraviolet light in which there was an alteration of mitochondria and a loss of cytoplasmic homogeneity. The data imply that the trifluoperazine HCI photoproduct had an adverse effect on cellular transport mechanisms. The study also further demonstrates that value of the corneal endothelial cell model for identifying the physiological and anatomical changes occurring in photo‐induced toxic reactions.