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EFFECT OF INACTIVATION OF THE OXYGEN‐EVOLVING SYSTEM ON THE THERMOLUMINESCENCE OF ISOLATED CHLOROPLASTS
Author(s) -
Rózsa Zsuzsa,
Demeter Sándor
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb09492.x
Subject(s) - thermoluminescence , chemistry , chaotropic agent , oxygen evolution , oxygen , oxygen deficiency , incubation , enzyme , enzyme assay , inorganic chemistry , chloroplast , photochemistry , biochemistry , luminescence , materials science , organic chemistry , optoelectronics , electrode , electrochemistry , gene
— The effect of inactivation of water‐splitting enzyme on the thermoluminescence of isolated chloroplasts was investigated. The inhibitory treatments used included Tris‐washing, alkaline pH in the presence of the uncoupler gramicidin, incubation with a high concentration of magnesium ions and different chaotropic agents. It was found that inhibition of oxygen evolution resulted in the disappearance of the main thermoluminescence band at +20°C. The A band which appears at — 10°C and has been related to the S 4 state of the water‐splitting enzyme (Inoue, 1981) was not considerably affected by the inhibition of oxygen evolution. The results presented here indicate that the participation of the S 4 state of water‐splitting enzyme in the generation of the A band should be reinvestigated.