Premium
EARLY EFFECTS OF NEAR‐ULTRAVIOLET RADIATION ON YEAST CELLS: CHANGES IN CELL SIZE
Author(s) -
Watanabe Toshiki,
Ito Atsushi,
Ito Takashi
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb04422.x
Subject(s) - irradiation , yeast , ultraviolet , centrifugation , exponential growth , shrinkage , radiation , suspension culture , suspension (topology) , biophysics , ultraviolet radiation , differential centrifugation , fluence , chemistry , coulter counter , biology , materials science , chromatography , optics , radiochemistry , cell culture , biochemistry , physics , optoelectronics , microbiology and biotechnology , composite material , mathematics , genetics , quantum mechanics , homotopy , nuclear physics , pure mathematics
Exponentially growing yeast cells showed a drastic shrinkage when irradiated in water suspension (but not in buffer) with broad‐band near‐UV radiation. This was clearly seen soon after irradiation in size‐distribution curves measured by a Coulter counter. This early shrinkage was observed at a near‐UV fluence where the survival enters a region of exponential decline after a large shoulder. We further observed the formation of a distinct band in a density‐gradient‐centrifugation profile, presumably corresponding to the near‐UV‐affected cells. A survival test showed that the cells making up this band were dead.