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BLOCKAGE OF RESTRICTION ENDONUCLEASE CLEAVAGE BY THYMINE DIMERS
Author(s) -
Hall Robert K.,
Larcom L. L.
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb04398.x
Subject(s) - restriction enzyme , cleavage (geology) , thymine , dna , endonuclease , recognition sequence , plasmid , chemistry , dimer , biology , microbiology and biotechnology , stereochemistry , biochemistry , paleontology , organic chemistry , fracture (geology)
Viral DNAs were subjected to 254 nm irradiation and then digested with type II restriction endonucleases. At the fluences used, irradiation inhibited cleavage by nucleases which recognize sites containing neighboring thymines. Cleavage by endonucleases with other recognition sequences was not affected. In viral and plasmid DNAs, this effect could be used to study thymine dimer formation at a few specific, mapped sites of defined base sequence.