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FORMATION OF NON‐VIABLE SPORES OF DICTYOSTELIUM DISCOIDEUM BY UV‐IRRADIATION AND CAFFEINE
Author(s) -
Ohnishi Takeo,
Hazama Masumi,
Okaichi Kumio,
Nozu Keiichi
Publication year - 1982
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1982.tb04386.x
Subject(s) - spore , dictyostelium discoideum , germination , irradiation , mycetozoa , caffeine , biology , microbiology and biotechnology , strain (injury) , biophysics , population , chemistry , botany , biochemistry , anatomy , physics , demography , sociology , nuclear physics , gene , endocrinology
We have characterized the spores formed from amoeboid cells of the wild type strain of Dictyostelium discoideum after UV‐irradiation. Cell differentiation in the presence of caffeine after a fluence of 300 J/m 2 resulted in a population of spores which was 98% non‐viable. The UV‐irradiation did not affect the conversion of the spores to swollen spores but did affect the conversion of swollen spores to amoeboid cells. When the germination of the spores was done without caffeine, we detected only a small effect on conversion of swollen spores to amoeboid cells and on the beginning of growth. On the other hand, in the presence of caffeine, the spores had a remarkable delay in both. It was also shown that few, if any, pyrimidine dimers exist in the DNA of the non‐viable spores. Possible mechanisms of formation of non‐viable spores are discussed.